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KMID : 0380420160400020071
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Journal of Prventive Veterinary Medicine
2016 Volume.40 No. 2 p.71 ~ p.79
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Antimicrobial resistance and molecular characterization of Clostridium perfringens isolated from chicken
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Lee Young-Ju
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Abstract
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Clostridium perfringens (C. perfringens) induced necrotic enteritis (NE) and related subclinical disease has become economically significant problems for the broiler industry. The objectives of this study were to determine the toxinotype and the antimicrobial susceptibility of C. perfringens isolated from the intestine of NE-suspicious and clinically healthy chickens in Korea. All of the 110 isolates belong to C. perfringens type A. In addition, these strains harbored the luxS, colA, virS, cpb2, netB and tpeL genes in high percentage order. They do not harbor the cpe gene encoding enterotoxin production. Our results suggest the important roles of the several toxin genes in the pathogenesis of NE except α-toxin, previously thought to be the major virulence factor. In the antimicrobial susceptibility test of 13 antimicrobial agents, none of them is completely susceptible in this study. Remarkably NE-suspicious chickens have higher resistant value than health ones in all of antimicrobial agents. The presence of tetracycline-resistance genes were also tested to analyze the correlation of genotype and phenotype.
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KEYWORD
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Antimicrobial resistance, chickens, Clostridium perfringens, necrotic enteritis, toxinotypes
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